Pci neo vector promega

Article Snippet: The cDNA coding for FGFR2c was amplified by pMD18T-simple FGFR2c (HG10824-M, Sino Biological Inc., Beijing, China) and subcloned in pCI-neo expression vector using standard procedures (Promega, Madison, WI) and the resulting pCI-neo FGFR2c construct was verified by …

E1711 Plasmid pCI-neo:alpha4-eGFP from Dr. Henry Lester's lab contains the insert alpha4-eGFP and is published in J Gen Physiol. 2011 Jan;137(1):59-79. This plasmid is available through Addgene. Name: pCI-neo: Type: plasmid: Supplier: Promega: Description: The pCI-neo Mammalian Expression Vector carries the human cytomegalovirus (CMV) immediate-early enhancer/promoter region to promote constitutive expression of cloned DNA inserts in mammalian cells.The pCI-neo Vector can be used for transient or stable expression by selecting transfected cells with the antibiotic G-418. Plasmid pCI-neo-RL-ZEB1 from Dr. Greg Goodall's lab contains the insert 3'UTR of ZEB1 and is published in Nat Cell Biol. 2008 May;10(5):593-601.

15.12.2020

3. Vector Components 3.A. Enhancer/Promoter Regions The CMV immediate-early enhancer/promoter region present in the pCI-neo The pCI-neo Mammalian Expression Vector carries the human cytomegalovirus (CMV) immediate-early enhancer/promoter region to promote strong, constitutive expression of cloned DNA inserts in mammalian cells. A β-globin/IgG chimeric intron located downstream from the enhancer/promoter region can further increase expression. The pCI-neo Mammalian Expression Vector carries the human cytomegalovirus (CMV) immediate-early enhancer/promoter region to promote strong, constitutive expression of cloned DNA inserts in mammalian cells.

Promega Corporation is a worldwide leader in applying biochemistry and molecular biology to the development of innovative, high-value products for the life sciences. The Promega mission statement is: To be the most responsive supplier of biological reagents and reagent systems used in research and applied technology applications worldwide.

The pCI-neo Vector can be used for transient or stable expression by selecting transfected cells with the antibiotic G-418. pCI-neo Mammalian Expression Vectorは、哺乳動物細胞における安定した遺伝子発現に最適な発現ベクターです。抗生物質G418を用いてトランスフェクト細胞を選択すれば、一過性の発現または安定な発現に利用できます。 Promega pCI and pCI-neo Mammalian Expression Vectors Contains the CMV immediate-early Promega Corporation is a worldwide leader in applying biochemistry and molecular biology to the development of innovative, high-value products for the life sciences. The Promega mission statement is: To be the most responsive supplier of biological reagents and reagent systems used in research and applied technology applications worldwide. Promega pCI and pCI-neo Mammalian Expression Vectors Contains the CMV immediate-early enhancer/promoter region and is designed to promote constitutive expression of cloned DNA inserts in mammalian cells.

Many Promega Vectors are reporter vectors containing various combinations of luciferase genes (firefly, Renilla, NanoLuc luciferase) with other genetic elements. The luminescence signal from these reporter vectors is used as an indicator of the activity of any gene/DNA element fused to the reporter.

pCMVT n T™ Vector contains a CMV enhancer/promoter region, which enables strong constitutive expression in many cell types, while RNA phage promoters enable RNA synthesis in vitro. L5620, L5610 Cytokine Signaling Pathway Analysis Firefly Luciferase Vectors The pCI-neo Mammalian Expression Vector carries the human cytomegalovirus (CMV) immediate-early enhancer/promoter region to promote constitutive expression of cloned DNA inserts in mammalian cells. The pCI-neo Vector contains the neomycin phosphotransferase gene, a selectable marker for mammalian cells. Promega pCI and pCI-neo Mammalian Expression Vectors. Contains the CMV immediate-early The pCI-neo Mammalian Expression Vector carries the human cytomegalovirus (CMV) immediate-early enhancer/promoter region to promote strong, constitutive expression of cloned DNA inserts in mammalian cells. A β-globin/IgG chimeric intron located downstream from the enhancer/promoter region can further increase expression. pCI-neo.

The pCI-neo Mammalian Expression Vector carries the human cytomegalovirus (CMV) immediate-early enhancer/promoter region to promote strong, constitutive expression of cloned DNA inserts in mammalian cells. A β-globin/IgG chimeric intron located downstream from the enhancer/promoter region can further increase expression. pCI-neo Mammalian Expression Vectorは、哺乳動物細胞における安定した遺伝子発現に最適な発現ベクターです。抗生物質G418を用いてトランスフェクト細胞を選択すれば、一過性の発現または安定な発現に利用できます。 The pCI Expression Vector contains the human cytomegalovirus (CMV) major immediate-early gene enhancer/promoter region. A β-globin/IgG chimeric intron located downstream of the enhancer/promoter region can further increase expression in the pCI Vector.

Plasmid pCI-neo Flag HAUSP from Dr. Bert Vogelstein's lab contains the insert HAUSP and is published in Cell Cycle. 2004 Jun . 3(6):689-92. This plasmid is available through Addgene. KpnI sites of the pCI-neo vector (Promega).

This vector also contains the neomycin phosphotransferase gene, a selectable marker for stable transfection of mammalian cells. The pCI-neo Vector can be used for transient or stable expression by selecting transfected cells with the antibiotic G-418. Multiple cloning sites exist for easy insertion of cDNA. References The pCI Expression Vector contains the human cytomegalovirus (CMV) major immediate-early gene enhancer/promoter region. A β-globin/IgG chimeric intron located downstream of the enhancer/promoter region can further increase expression in the pCI Vector. Product Availability Update. Promega Manufacturing and Delivery Systems continue to be fully operational during the COVID-19 outbreak.

Vector database is a digital collection of vector backbones assembled from publications and Convenient expression of cloned genes in vitro or in vivo. pCMVT n T™ Vector contains a CMV enhancer/promoter region, which enables strong constitutive expression in many cell types, while RNA phage promoters enable RNA synthesis in vitro. L5620, L5610 Cytokine Signaling Pathway Analysis Firefly Luciferase Vectors The pCI-neo Mammalian Expression Vector carries the human cytomegalovirus (CMV) immediate-early enhancer/promoter region to promote constitutive expression of cloned DNA inserts in mammalian cells. The pCI-neo Vector contains the neomycin phosphotransferase gene, a selectable marker for mammalian cells.

The pCI-neo Vector can be used for transient expression or for stable expression by selecting transfected cells with the antibiotic G-418. Return to this vector's summary. LOCUS CVU47120 5474 bp DNA circular SYN 20-FEB-1996 DEFINITION Cloning vector pCI-neo, mammalian expression vector… pCI-neo Mammalian Expression Vector. DNA cloned into this vector is constitutively expressed in mammalian cells; use the neomycin resistance gene for stable expression. E1841. pAdVAntage™ Vector.

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Restriction digestion of NS4B gblock and pCI-neo vector (Promega, Cat# E1841) was performed by using XhoI and XbaI restriction enzymes. Following restriction,

pCI-neo Mammalian Expression Vectorは、哺乳動物細胞における安定した遺伝子発現に最適な発現ベクターです。抗生物質G418を用いてトランスフェクト細胞を選択すれば、一過性の発現または安定な発現に利用できます。 Promega pCI and pCI-neo Mammalian Expression Vectors Contains the CMV immediate-early pCI-neo Mammalian cell expression vector with the CMV promoter and a neomycin (G418) resistance marker. To see this sequence with restriction sites, features, and translations, please download SnapGene or the free SnapGene Viewer. Contains a neomycin resistance gene and the CMV immediate-early enhancer/promoter region to promote constitutive expression of cloned DNA inserts in mammalian cells. Transfected cells can be selected with G-418.In molecular cloning, vectors are DNA molecules used to artificially deliver foreign genetic material into another cell.